Molecular AND logic gate for multiple single-nucleotide mutations detection based on CRISPR/Cas9n system-trigged signal amplification.
Identifieur interne : 000121 ( Main/Exploration ); précédent : 000120; suivant : 000122Molecular AND logic gate for multiple single-nucleotide mutations detection based on CRISPR/Cas9n system-trigged signal amplification.
Auteurs : Weidan Chang [République populaire de Chine] ; Weipeng Liu [République populaire de Chine] ; Haoran Shen [République populaire de Chine] ; Sisi Chen [République populaire de Chine] ; Peizhen Liao [République populaire de Chine] ; Yingju Liu [République populaire de Chine]Source :
- Analytica chimica acta [ 1873-4324 ] ; 2020.
Abstract
Precise detection of single-nucleotide mutations (SNMs) is extremely important in various biomedical applications, but the simultaneous detection of multiple SNMs remains a great challenge. Herein, we developed a new method based on CRISPR/Cas9 system for multiple SNMs detection. The CRISPR/Cas9 system transduces the nucleic acid into an intermediate trigger to initiate the isothermal amplification reaction and further form fluorescence signals. According to this strategy, we established nucleic acid bio-computing operations-molecule logic gate in simultaneous distinction of the genetic locus. We demonstrate that the fluorescence signals generated from different input combinations can be used to discriminate the multiple genetic locus, and the molecular logic gate has great potential in single-base mismatch detection. In addition, the successful assay of real samples indicates that the novel strategy could further adapt for the pathogenic monitoring and biomedical research.
DOI: 10.1016/j.aca.2020.03.058
PubMed: 32334681
Affiliations:
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Electronic address: weipeng_liu@scau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Shen, Haoran" sort="Shen, Haoran" uniqKey="Shen H" first="Haoran" last="Shen">Haoran Shen</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Chen, Sisi" sort="Chen, Sisi" uniqKey="Chen S" first="Sisi" last="Chen">Sisi Chen</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Liao, Peizhen" sort="Liao, Peizhen" uniqKey="Liao P" first="Peizhen" last="Liao">Peizhen Liao</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Liu, Yingju" sort="Liu, Yingju" uniqKey="Liu Y" first="Yingju" last="Liu">Yingju Liu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory of Lingnan Modern Agriculture, Guangzhou 510642, China. Electronic address: liuyingju@hotmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory of Lingnan Modern Agriculture, Guangzhou 510642</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author></analytic><series><title level="j">Analytica chimica acta</title><idno type="eISSN">1873-4324</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Precise detection of single-nucleotide mutations (SNMs) is extremely important in various biomedical applications, but the simultaneous detection of multiple SNMs remains a great challenge. Herein, we developed a new method based on CRISPR/Cas9 system for multiple SNMs detection. The CRISPR/Cas9 system transduces the nucleic acid into an intermediate trigger to initiate the isothermal amplification reaction and further form fluorescence signals. According to this strategy, we established nucleic acid bio-computing operations-molecule logic gate in simultaneous distinction of the genetic locus. We demonstrate that the fluorescence signals generated from different input combinations can be used to discriminate the multiple genetic locus, and the molecular logic gate has great potential in single-base mismatch detection. In addition, the successful assay of real samples indicates that the novel strategy could further adapt for the pathogenic monitoring and biomedical research.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32334681</PMID><DateRevised><Year>2020</Year><Month>04</Month><Day>26</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4324</ISSN><JournalIssue CitedMedium="Internet"><Volume>1112</Volume><PubDate><Year>2020</Year><Month>May</Month><Day>22</Day></PubDate></JournalIssue><Title>Analytica chimica acta</Title><ISOAbbreviation>Anal Chim Acta</ISOAbbreviation></Journal><ArticleTitle>Molecular AND logic gate for multiple single-nucleotide mutations detection based on CRISPR/Cas9n system-trigged signal amplification.</ArticleTitle><Pagination><MedlinePgn>46-53</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0003-2670(20)30379-2</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.aca.2020.03.058</ELocationID><Abstract><AbstractText>Precise detection of single-nucleotide mutations (SNMs) is extremely important in various biomedical applications, but the simultaneous detection of multiple SNMs remains a great challenge. Herein, we developed a new method based on CRISPR/Cas9 system for multiple SNMs detection. The CRISPR/Cas9 system transduces the nucleic acid into an intermediate trigger to initiate the isothermal amplification reaction and further form fluorescence signals. According to this strategy, we established nucleic acid bio-computing operations-molecule logic gate in simultaneous distinction of the genetic locus. We demonstrate that the fluorescence signals generated from different input combinations can be used to discriminate the multiple genetic locus, and the molecular logic gate has great potential in single-base mismatch detection. In addition, the successful assay of real samples indicates that the novel strategy could further adapt for the pathogenic monitoring and biomedical research.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Weidan</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China; The Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Weipeng</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China. Electronic address: weipeng_liu@scau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Haoran</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Sisi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liao</LastName><ForeName>Peizhen</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Yingju</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Key Laboratory for Biobased Materials and Energy of Ministry of Education, Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory of Lingnan Modern Agriculture, Guangzhou 510642, China. Electronic address: liuyingju@hotmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Anal Chim Acta</MedlineTA><NlmUniqueID>0370534</NlmUniqueID><ISSNLinking>0003-2670</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">CRISPR/Cas9 system</Keyword><Keyword MajorTopicYN="N">Molecular logic gate</Keyword><Keyword MajorTopicYN="N">Multiplex SNMs</Keyword><Keyword MajorTopicYN="N">Phytophthora infestans</Keyword><Keyword MajorTopicYN="N">Signal amplification</Keyword></KeywordList><CoiStatement>Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>11</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>03</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32334681</ArticleId><ArticleId IdType="pii">S0003-2670(20)30379-2</ArticleId><ArticleId IdType="doi">10.1016/j.aca.2020.03.058</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Chang, Weidan" sort="Chang, Weidan" uniqKey="Chang W" first="Weidan" last="Chang">Weidan Chang</name></region><name sortKey="Chen, Sisi" sort="Chen, Sisi" uniqKey="Chen S" first="Sisi" last="Chen">Sisi Chen</name><name sortKey="Liao, Peizhen" sort="Liao, Peizhen" uniqKey="Liao P" first="Peizhen" last="Liao">Peizhen Liao</name><name sortKey="Liu, Weipeng" sort="Liu, Weipeng" uniqKey="Liu W" first="Weipeng" last="Liu">Weipeng Liu</name><name sortKey="Liu, Yingju" sort="Liu, Yingju" uniqKey="Liu Y" first="Yingju" last="Liu">Yingju Liu</name><name sortKey="Shen, Haoran" sort="Shen, Haoran" uniqKey="Shen H" first="Haoran" last="Shen">Haoran Shen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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